The project aims to develop and commercialize novel 3D spheroid-co-cultures of human hepatocytes and liver non-parenchymal cells from the same human liver donors including:
- 3D spheroid co-cultures (to predict drug metabolism and hepatotoxicity);
- Pharmacogenetic profiles of donors for studying inter-individual genetic variation;
- Cryopreservation methods for spheroids;
- New models to analyse drug hepatotoxicity in diseased livers (steatosis, fibrosis, cholestasis) and validate new drugs.